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Image Search Results
Journal: BMC Molecular and Cell Biology
Article Title: Estrogen suppresses SOX9 and activates markers of female development in a human testis-derived cell line
doi: 10.1186/s12860-020-00307-9
Figure Lengend Snippet: ERα immunofluorescence staining in NT2/D1 cells after 48 h of culture. ERα (green) was primarily cytoplasmic in control cells. EE2 treatment at both 10 nM and 100 nM led to a significant increase in nuclear ERα. Top panel shows ERα, middle panel shows DAPI, bottom panel shows a merge. Nuclei stained with DAPI; magenta. Scale bar 5 μm
Article Snippet: Fixed cells were exposed to a one-hour blocking step in 10% horse serum in 0.1% TritonX in PBS, before being stained with anti-SOX9 (ab5535; Millipore; 1:500) or anti-ERα (ab37438; Abcam; 1:500) and the
Techniques: Immunofluorescence, Staining, Control
Journal: BMC Molecular and Cell Biology
Article Title: Estrogen suppresses SOX9 and activates markers of female development in a human testis-derived cell line
doi: 10.1186/s12860-020-00307-9
Figure Lengend Snippet: SOX9 immunofluorescence staining in NT2/D1 cells after 48 h of culture. SOX9 (green) was primarily nuclear (nuclei marked by DAPI; magenta) in the control cells. EE2 treatment at both 10 nM and 100 nM led to a greater amount of SOX9 in the cytoplasm. Top panel shows SOX9, middle panel shows DAPI, bottom panel shows a merge. Scale bar 5 μm
Article Snippet: Fixed cells were exposed to a one-hour blocking step in 10% horse serum in 0.1% TritonX in PBS, before being stained with anti-SOX9 (ab5535; Millipore; 1:500) or anti-ERα (ab37438; Abcam; 1:500) and the
Techniques: Immunofluorescence, Staining, Control
Journal: BMC Molecular and Cell Biology
Article Title: Estrogen suppresses SOX9 and activates markers of female development in a human testis-derived cell line
doi: 10.1186/s12860-020-00307-9
Figure Lengend Snippet: SOX9 immunofluorescence staining in NT2/D1 cells after 96 h of culture. SOX9 (green) was primarily nuclear (nuclei marked by DAPI; magenta) in the control cells. EE2 treatment at both 10 nM and 100 nM led to a greater amount of SOX9 in the cytoplasm. Top panel shows SOX9, middle panel shows DAPI, bottom panel shows a merge. Scale bar 5 μm
Article Snippet: Fixed cells were exposed to a one-hour blocking step in 10% horse serum in 0.1% TritonX in PBS, before being stained with anti-SOX9 (ab5535; Millipore; 1:500) or anti-ERα (ab37438; Abcam; 1:500) and the
Techniques: Immunofluorescence, Staining, Control